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OBJECTIVE@#To investigate the therapeutic effect of Epothilone D on traumatic optic neuropathy (TON) in rats.@*METHODS@#Forty-two SD rats were randomized to receive intraperitoneal injection of 1.0 mg/kg Epothilone D or DMSO (control) every 3 days until day 28, and rat models of TON were established on the second day after the first administration. On days 3, 7, and 28, examination of flash visual evoked potentials (FVEP), immunofluorescence staining and Western blotting were performed to examine the visual pathway features, number of retinal ganglion cells (RGCs), GAP43 expression level in damaged axons, and changes of Tau and pTau-396/404 in the retina and optic nerve.@*RESULTS@#In Epothilone D treatment group, RGC loss rate was significantly decreased by 19.12% (P=0.032) on day 3 and by 22.67% (P=0.042) on day 28 as compared with the rats in the control group, but FVEP examination failed to show physiological improvement in the visual pathway on day 28 in terms of the relative latency of N2 wave (P=0.236) and relative amplitude attenuation of P2-N2 wave (P=0.441). The total Tau content in the retina of the treatment group was significantly increased compared with that in the control group on day 3 (P < 0.001), showing a consistent change with ptau-396/404 level. In the optic nerve axons, the total Tau level in the treatment group was significantly lower than that in the control group on day 7 (P=0.002), but the changes of the total Tau and pTau-396/404 level did not show an obvious correlation. Epothilone D induced persistent expression of GAP43 in the damaged axons, detectable even on day 28 of the experiment.@*CONCLUSION@#Epothilone D treatment can protect against TON in rats by promoting the survival of injured RGCs, enhancing Tau content in the surviving RGCs, reducing Tau accumulation in injured axons, and stimulating sustained regeneration of axons.
Subject(s)
Animals , Rats , Disease Models, Animal , Epothilones , Evoked Potentials, Visual , Nerve Regeneration/physiology , Optic Nerve Injuries/metabolism , Rats, Sprague-Dawley , Retinal Ganglion Cells/physiologyABSTRACT
Purpose@#Systemic inflammatory response is a critical factor that promotes the initiation and metastasis of malignancies including pancreatic cancer (PC). This study was designed to determine and compare the prognostic value of neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and fibrinogen-to-albumin ratio (FAR) in resectable PC and locally advanced or metastatic PC. @*Materials and Methods@#Three hundred fifty-three patients with resectable PC and 807 patients with locally advan-ced or metastatic PC were recruited in this study. These patients were classified into a training set (n=758) and a validation set (n=402). Kaplan-Meier survival plots and Cox proportional hazards regression models were used to analyze prognosis. @*Results@#Overall survival (OS) was significantly better for patients with resectable PC with low preoperative PLR (p=0.048) and MLR (p=0.027). Low FAR, MLR, NLR (p < 0.001), and PLR (p=0.003) were significantly associated with decreased risk of death for locally advanced or metastatic PC patients. FAR (hazard ratio [HR], 1.522; 95% confidential interval [CI], 1.261 to 1.837; p < 0.001) and MLR (HR, 1.248; 95% CI, 1.017 to 1.532; p=0.034) were independent prognostic factors for locally advanced or metastatic PC. @*Conclusion@#The prognostic roles of FAR, MLR, NLR, and PLR in resectable PC and locally advanced or metastatic PC were different. FAR showed the most prognostic power in locally advanced or metastatic PC. Low FAR was positively correlated with OS in locally advanced or metastatic PC, which could be used to predict the prognosis.
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OBJECTIVE: To study the effects and mechanisms of danhong injection on cerebral ischemia reperfusion injury in rats with hyperlipidemia. METHODS: Hyperlipidemia model was established by feeding SD rats with high-fat diet for 6 weeks. Then hyperlipidemia rats were randomly divided into sham operation group, model group and danhong injection low (0.5 mL•kg-1), medium (1.0 mL•kg-1) and high (2.0 mL•kg-1) dose groups with six rats for each group. Danhong injection groups were injected for 7 consecutive days before the establishment of the model of middle cerebral artery occlusion (MCAO), and the corresponding dose was given once at 1 h before modeling and 6 h after reperfusion. After 24 h of reperfusion, rats were evaluated in terms of neurological function. The changes of histopathology were observed in the cerebral cortex of ischemic rats by HE staining; RT-PCR was used to detect the mRNA expressions of Nox2, Rac1, ASC, NLRP3, caspase-1, IL-1β and IL-18 in cerebral ischemia area. In addition, the content of triglyceride (TG) and total cholesterol (TC) in serum were detected by the kits. The expression of NLRP3 protein was evaluated by immunohistochemical. RESULTS: Compared with MCAO group, Danhong injection groups improved the reperfusion injury of cerebral ischemia in varying degrees, significantly reduced the mRNA expression of Nox2, Rac1, ASC, NLRP3, caspase-1, IL-1β and IL-18, increased the neurological score of MCAO rats, and reduced the contents of TC and TG besides the expression of NLRP3 protein. CONCLUSION: Danhong injection could effectively regulate blood lipid level and play a obvious protective effect on the reperfusion injury in MCAO rats with hyperlipidemia. The mechanism might be related to the inhibition of the formation of NLRP3 inflammatory body and the decrease of the inflammation-related factors expression of caspase-1, IL-1β and IL-18.
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OBJECTIVE@#To evaluate the short-term curative effects of ARCH titanium plate fixation combined with expansive single open-door laminoplasty (EOLP) in treating cervical spondylotic myelopathy (CSM).@*METHODS@#EOLP with ARCH titanium plate as internal fixation material was applied in 32 patients with CSM from January to December 2016. There were 23 males and 9 females with an average age of 64.5 years ranging from 39 to 82 years. The course of disease ranged from 6 to 24 months with an average of 13.1 months. The clinical efficacy was evaluated by Japanese Orthopaedic Association (JOA) scoring method, which included upper and lower limb motor function, limb sensory function and bladder function. The sagittal diameter of the narrowest segment of vertebral canal was measured by imaging data before operation and 6 months after operation, and the improvement rate was calculated to determine the decompression effect.@*RESULTS@#All the patients were followed up from 6 to 20 months with an average of 12.2 months. Preoperative symptoms of 32 patients were improved to varying degrees, the JOA score increased from 9.78±1.34 before operation to 12.94±1.16 at 6 months after operation, the improvement rate of JOA was(44.09±11.06)% (<0.01). The spinal canal was significantly enlarged, the sagittal diameter of the narrowest vertebral canal was increased from (8.47±0.60) mm preoperatively to (12.51±0.78) mm 6 months postoperatively, the improvement rate was (48.27±11.81)% (<0.01). No loosening, displacement, rupture or "re-closure" of the internal fixator was found during the follow-up.@*CONCLUSIONS@#ARCH titanium plate fixation combined with EOLP in the treatment of CSM can significantly reduce the possibility of "re-closure" and other related postoperative complications and the short-term clinical efficacy is satisfactory.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Bone Plates , Cervical Vertebrae , Laminoplasty , Retrospective Studies , Spinal Cord Diseases , Titanium , Treatment OutcomeABSTRACT
Objective To compare the clinical effect of minimally invasive surgery transforaminal lumbar interbody fu-sion(MIS-TLIF)and posterior lumbar interbody fusion(PLIF)in the treatment of recurrent lumbar intervertebral disc hernia-tion(LIDH). Methods Twenty-nine patients with recurrent LIDP were selected from May 2014 to May 2016 in Weifang Peo-ple's Hospital and the clinical data were analyzed retrospectively. Thirteen patients were given MIS-TLIF(MIS-TLIF group) and sixteen patients were given PLIF(PLIF)group. The operative incision length,intraoperative bleeding volume,postoperative drainage volume,hospitalization time and complications were compared between the two groups. The lumbar function was evalu-ated with the Japanese Orthopaedic Association(JOA)score standard,and the clinical effect was compared between the two groups according to the modified Macnab standard one year after treatment. Results The operativeincision length,intraopera-tive bleeding volume,postoperative drainage volumeand hospitalization time in MIS-TLIF group were significantly less than tho-sein PLIF group (P < 0. 05). The preoperative JOA score of lumbar function in PLIF group and MIS-TLIF group was 7. 9 ± 1. 9 and 8. 0 ± 1. 6 respectively,it was 24. 0 ± 2. 7 and 24. 2 ± 2. 5 respectively at one year after treatment,there was no significant-difference in the JOA score between the two groups before and one year after operation (P > 0. 05). The JOA score atone year after operation was significantly higher than that before operation in the two groups (P < 0. 05). According to the modified Macnab standard one year after treatment,the fineness rate of the patients in PLIF group was 87. 50％(14 / 16),the fineness rate of the patients in the MIS-TLIF group was 84. 62％(11 / 13). There was no significant difference in the fineness rate be-tweenthe two groups (χ2 = 1. 380,P > 0. 05). The incidence of postoperative complications in the MIS-TLIF group and PLIF group was 7. 7％(1/ 13)and 6. 3％ (1/ 16)respectively,there was no significant difference in the incidence of postoperative complications between the two groups (χ2 = 0. 020,P > 0. 05). There were 8 cases (61. 5％)with gradeⅠfusion and 5 cases (38. 5％)with gradeⅡfusion in MIS-TLIF group,there were 9(56. 3％)with gradeⅠfusion and 7(43. 8％)with gradeⅡfu-sion,there was no significant difference in the constituent ratio with gradeⅠandⅡfusion between the two groups (χ2 = 0. 080, P >0. 05). Conclusion MIS-TLIF in treatment of recurrent LIDH has the advantages of less incision,less intraoperative bleed-ing,less postoperative drainage and shorter hospitalization time;and the clinical effect of MIS-TLIF is similar to that of PLIF.
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Objective To evaluate the bond strength of GuttaFlow sealers to root canal walls after final rinse with two novel fi nal irrigation regimens, QMiX and 17%EDTA+0.2%Cetrimide (CTR). Methods Thirty single-canal teeth were prepared chemomechanically using 5.25% sodium hypochlorite (NaOCl) as root canal irrigants. The teeth were then randomly distributed into three groups(n=10)according to the final irrigation protocol:QMiX group,17% EDTA followed by CTR group and control group(normal saline,NS).After the filling with GuttaFlow using a lentulo spiral,the roots were transversally sectioned to obtain 2 mm thick dentinal slices.Then a push-out bond strength test was carried out,and failure mode was determined at×24 magnification.Results The push-out bond strength was significantly higher in QMiX group than that of EDTA+CTR group and NS group(P<0.05),and no significant difference was observed between EDTA+CTR and NS groups(P>0.05).The failure patterns were mainly mixed.Conclusion The QMiX,as the final irrigant,can improve the bond strength of silicone-based sealer GuttaFlow.
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The prevalence of hypertensive disorders in China was much higher than that in the United States. Considering the large population with wide geographic area of China, we aimed to add more information regarding the risk factors for hypertensive disorders of pregnancy. A case-control study was performed on 373 hypertensive cases and 507 normotensive controls. Participants were recruited from 2008 to 2014 in Yichang Maternal and Child Health Care Center in Hubei province and Anyang Maternal and Child Health Care Hospital in Henan province, China. Socio-demographic factors, family- related factors, pregnancy-associated factors, factors related to daily life behaviors and psychosocial factors were investigated with respect to hypertensive disorders in pregnancy through well-designed questionnaire. Chi-square test, t-test, univariate logistic regression analysis, and multivariate logistic regression analysis were used to find the possible risk factors behind hypertensive disorders in pregnancy. The results showed that family history of cardiovascular diseases (OR=6.18, 95% CI, 2.37 to 16.14), history of pregnancy-induced hypertension (OR=16.64, 95% CI, 5.74 to 48.22), low maternal educational level (OR=2.81, 95% CI, 1.30 to 6.04), and poor relationship with their parents-in-law (OR=3.44, 95% CI, 1.55 to 7.59) had statistically significant associations with hypertensive disorders in pregnancy through multivariate logistic regression analysis. Increased maternal age, increased pre-pregnancy body mass index, living in rural area, low paternal education level, family history of hypertension, passive smoking one year before and/or in pregnancy, and poor sleeping quality were significantly associated with hypertensive disorders in pregnancy from univariate logistic regression analysis while the associations became uncertain when they were entered for multivariate logistic regression analysis. It was concluded that family history of cardiovascular diseases, history of pregnancy-induced hypertension, low maternal educational level, and poor relationship with their parents-in-law were independent risk factors for hypertensive disorders among Chinese pregnant women.
Subject(s)
Adult , Female , Humans , Pregnancy , Young Adult , China , Epidemiology , Hypertension , Epidemiology , Pregnancy Complications, Cardiovascular , Epidemiology , Risk FactorsABSTRACT
This study is designed to explore the possible effects of Hemerocallis citrina baroni flavonids (HCBF) on liver fibrosis induced by CCl4 in rats. The liver fibrosis model was induced by CCl4, and HCBF were administered by gastric perfusion at 25 and 50 mg x kg(-1) qd for 50 days, while the contents of alanine transaminase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), superoxide dismutase (SOD), maleic dialdehyde (MDA) and transforming growth factor-β1 (TGF-β1) were measured and the contents of PINP were measured in liver tissue, and the expression of TGF-β1 were observed by immunohistochemisty and Western blot. The pathological changes of liver tissue were examined by HE. The results showed that HCBF (25, 50 mg x kg(-1)) improved the liver function significantly through reducing the level of ALT, AST, GGT and ALP (P < 0.05 or P < 0.01), and increasing the content of SOD (P < 0.01), while reducing the content of MDA (P < 0.05 or P < 0.01), the expression of TGF-β1 (P < 0.05) and the content of PINP (P < 0.05). The results suggest that HCBF (25, 50 mg x kg(-1)) may inhibit the liver injury induced by CCl4 by decreasing the oxidative stress.
Subject(s)
Animals , Rats , Alanine Transaminase , Metabolism , Alkaline Phosphatase , Metabolism , Aspartate Aminotransferases , Metabolism , Carbon Tetrachloride , Drugs, Chinese Herbal , Pharmacology , Flavonoids , Pharmacology , Hemerocallis , Chemistry , Liver Cirrhosis , Drug Therapy , Malondialdehyde , Metabolism , Oxidative Stress , Plant Extracts , Pharmacology , Superoxide Dismutase , Metabolism , Transforming Growth Factor beta1 , Metabolism , gamma-Glutamyltransferase , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of IFN alpha on the expressions of Collagen I and TGF beta 1 in hepatic stellate cell activated by PDGF-BB.</p><p><b>METHODS</b>Hepatic stellate cells (rHSC-99) treated with IFN alpha of different concentration (0, 0.0125, 0.025, 0.050, 0.100, 0.200, 0.400 ng/ml). The cell viability of HSC was measured by MTT. The levels of Col-I mRNA and TGF beta 1 mRNA were measured by the quantitative reverse-transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>(1) When HSC was exposed in PDGF-BB, the cell viability of HSC (1.35 +/- 0.22) was higher than that of the control group (0.890 +/- 0.12) (F = 16.311, P less than 0.05), indicating that PDGF-BB can promote the cell viability of HSC. When HSC was exposed to both PDGF-BB and different concentration of IFN alpha (0.025, 0.05, 0.1, 0.2, 0.4 ng/ml), the cell viability of HSC (0.840 +/- 0.18, 0.450 +/- 0.15, 0.260 +/- 0.01, 0.330 +/- 0.07, 0.30 +/- 0.06) were lower than that of the control group (0.890 +/- 0.12) (F = 7.430, P less than 0.05), indicating that the cell viability of HSC was inhibited when HSC was exposed to both PDGF-BB and different concentrations of IFN alpha. Furthermore, within the range of 0.025 ng/ml to 0.1 ng/ml, the effect of IFN alpha was dose-dependent. (2). The relative expression values of Col-I mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (0.940 +/- 0.19, 0.610 +/- 0.12, 0.520 +/- 0.02), which were lower than those in the control group (1.410 +/- 0.01) (F = 127.921, P less than 0.05). The relative expression values of TGF beta 1 mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (1.180 +/- 0.06, 1.150 +/- 0.10, 1.390 +/- 0.04), again were lower than those in the control group (1.620 +/- 0.12) (F = 82.115, P less than 0.05). These results indicated that the expression of Col-I mRNA and TGF beta 1 mRNA was remarkably inhibited when HSC was exposed in both PDGF-BB and IFN alpha.</p><p><b>CONCLUSION</b>The cell viability of HSC and the expression of Col-I mRNA and TGF beta 1 mRNA is remarkably inhibited when HSC is exposed in both PDGF-BB and IFN alpha, and the inhibition is dose-dependent.</p>
Subject(s)
Animals , Rats , Cells, Cultured , Collagen Type I , Genetics , Metabolism , Dose-Response Relationship, Drug , Gene Expression , Hepatic Stellate Cells , Metabolism , Interferon-alpha , Pharmacology , Liver Cirrhosis , Metabolism , Pathology , Platelet-Derived Growth Factor , Pharmacology , Proto-Oncogene Proteins c-sis , RNA, Messenger , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Methods , Transforming Growth Factor beta1 , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To explore the separation and culture method of adult hepatocytes.</p><p><b>METHODS</b>The isolated adult hepatocytes were cultivated by RPMI 1640 medium at 37 degrees C in vitro. The characteristics of the growing hepatocytes were observed. Their synthesis of urea was detected. The transformation efficiency and density's change of lidocaine were analyzed.</p><p><b>RESULTS</b>Hepatocytes were successful separated from adult liver. And they were cultivated in common condition and hollow fiber reactor. The functional capacity of hepatocytes was for lidocaine metabolism and urea excretion.</p><p><b>CONCLUSION</b>The adult hepatocytes have been successful separated from liver. And they can be cultivated in common condition and hollow fiber reactor. And it could provide a great quantity and high activity of hepatocytes for bioartificial liver.</p>
Subject(s)
Animals , Humans , Cell Culture Techniques , Cells, Cultured , Hepatocytes , Metabolism , Liver , Cell Biology , Liver, Artificial , ResearchABSTRACT
<p><b>BACKGROUND</b>Numerous studies have reported a relationship between hepatitis C virus (HCV) genotype and the response to interferon therapy. Despite high sensitivity and specificity, genotyping methods can be performed only on HCV RNA positive samples. Serotyping might be a rapid and cost effective method for determining HCV genotypes, especially in patients with previously undetectable HCV RNA. In this study, an enzyme linked immunosorbent assay (ELISA) method for HCV serotyping with the genotype specific, synthetic peptides derived from HCV nonstructural 5a (NS5A) region was developed.</p><p><b>METHODS</b>Based on 45 sequences, representing HCV genotypes 1 - 6 from Genebank, we synthesised 305 overlapping 30-mer peptides within NS5A region at positions 2182 - 2343 of HCV. All peptides for antigenic reactivity were tested by enzyme immunoassay with 69 human sera with antiHCV positive representing genotype 1 - 6. Forty hepatitis C patient sera were serotyped using serotype specific, synthetic peptides and genotyped by sequencing analysis.</p><p><b>RESULTS</b>The correspondence of amino acids in HCV NS5A region with amino acids in positions 2182 - 2343 was very low among different genotype peptides. The highly conserved sequences were residues 2182 - 2211 (R1), 2272 - 2301 (R7) and 2302 - 2331 (R9): the highly variable 2212 - 2241 (R3) and 2257 - 2286 (R6). Using 305 peptides, antigenic regions were located in R3, R7 and R9. Eighteen peptides from highly conserved region representing genotypes 1 to 6 showed broad immunoreactivity with sera containing antibody to all HCV genotypes. Immunoreactivity of the peptides from highly variable region was stronger with similar genotype sera. Twelve unique peptides showed highly, genotype specific, reactivity with types 1 and 3 sera. Type 2 genotype specific peptides had cross reaction with type 3 serum. No type 4, 5 or 6 specific peptides were selected. The serotyping results showed high agreement with sequencing analysis.</p><p><b>CONCLUSIONS</b>The major antigenic regions in HCV NS5A region were at 2212 - 2241 (R3), 2272 - 2301 (R7) and 2302 - 2331 (R9). Eighteen peptides from highly conserved region show genotype independent, immunoreactivity, useful for antiHCV antibody test. Twelve peptides from highly variable region show genotype 1 and 3 dependent immunoreactivity, useful for determining HCV serotype, especially for patients with previously undetectable HCV RNA.</p>